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Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics
Liang WF(梁文峰); Zhao YL(赵玉良); Liu LQ(刘连庆); Wang YC(王越超); Dong ZL(董再励); Li WJ(李文荣); Li GB(李国宾); Xiao XB(肖秀斌); Zhang WJ(张伟京)
Department机器人学研究室
Source PublicationPLOS ONE
ISSN1932-6203
2014
Volume9Issue:3Pages:1-9
Indexed BySCI
WOS IDWOS:000332485800056
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China [61107043]; CAS-Croucher Joint Lab Scheme [9500011]
KeywordDielectrophoretic Odep Force Mechanical-properties Electric-field Cancer-cells Manipulation Microscopy Enrichment System Device Images
AbstractEarly stage detection of lymphoma cells is invaluable for providing reliable prognosis to patients. However, the purity of lymphoma cells in extracted samples from human patients' marrow is typically low. To address this issue, we report here our work on using optically-induced dielectrophoresis (ODEP) force to rapidly purify Raji cells' (a type of Burkitt's lymphoma cell) sample from red blood cells (RBCs) with a label-free process. This method utilizes dynamically moving virtual electrodes to induce negative ODEP force of varying magnitudes on the Raji cells and RBCs in an optically-induced electrokinetics (OEK) chip. Polarization models for the two types of cells that reflect their discriminate electrical properties were established. Then, the cells' differential velocities caused by a specific ODEP force field were obtained by a finite element simulation model, thereby established the theoretical basis that the two types of cells could be separated using an ODEP force field. To ensure that the ODEP force dominated the separation process, a comparison of the ODEP force with other significant electrokinetics forces was conducted using numerical results. Furthermore, the performance of the ODEP-based approach for separating Raji cells from RBCs was experimentally investigated. The results showed that these two types of cells, with different concentration ratios, could be separated rapidly using externally-applied electrical field at a driven frequency of 50 kHz at 20 V-pp. In addition, we have found that in order to facilitate ODEP-based cell separation, Raji cells' adhesion to the OEK chip's substrate should be minimized. This paper also presents our experimental results of finding the appropriate bovine serum albumin concentration in an isotonic solution to reduce cell adhesion, while maintaining suitable medium conductivity for electrokinetics-based cell separation. In short, we have demonstrated that OEK technology could be a promising tool for efficient and effective purification of Raji cells from RBCs.
Language英语
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS KeywordDIELECTROPHORETIC ODEP FORCE ; MECHANICAL-PROPERTIES ; ELECTRIC-FIELD ; CANCER-CELLS ; MANIPULATION ; MICROSCOPY ; ENRICHMENT ; SYSTEM ; DEVICE ; IMAGES
WOS Research AreaScience & Technology - Other Topics
Citation statistics
Cited Times:58[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/14753
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Li WJ(李文荣)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong
4.Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan
5.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
Recommended Citation
GB/T 7714
Liang WF,Zhao YL,Liu LQ,et al. Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics[J]. PLOS ONE,2014,9(3):1-9.
APA Liang WF.,Zhao YL.,Liu LQ.,Wang YC.,Dong ZL.,...&Zhang WJ.(2014).Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics.PLOS ONE,9(3),1-9.
MLA Liang WF,et al."Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics".PLOS ONE 9.3(2014):1-9.
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