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Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy
Yang YL(杨永良)1,2; Zeng, Bixi3,4; Sun, Zhiyong1,5; Esfahani, Amir Monemian6; Hou, Jing7; Jiao ND(焦念东)8; Liu LQ(刘连庆)8; Chen, Liangliang1; Basson, Marc D.3,4; Dong, Lixin1; Yang, Ruiguo6; Xi N(席宁)1,5
Department机器人学研究室
Source PublicationIEEE TRANSACTIONS ON NANOTECHNOLOGY
ISSN1536-125X
2019
Volume18Pages:509-517
Indexed BySCI ; EI
EI Accession number20192407029004
WOS IDWOS:000469366400001
Contribution Rank8
Funding OrganizationNebraska Center for Integrated Biomolecular Communication (NIH National Institutes of General Medical Sciences) [P20 GM113126] ; Nebraska Center for Nanomedicine [P30 GM127200] ; NSF [1826135]
KeywordBionanotechnology nanosensors force spectroscopy atomic force microscopy protein-protein interactions
AbstractIncreasingly targeted in drug discovery, protein-protein interactions challenge current high throughput screening technologies in the pharmaceutical industry. Developing an effective and efficient method for screening small molecules or compounds is critical to accelerate the discovery of ligands for enzymes, receptors, and other pharmaceutical targets. Here, we report developments of methods to increase the signal-to-noise ratio for screening protein-protein interactions using atomic force microscopy (AFM) force spectroscopy. We have demonstrated the effectiveness of these developments on detecting the binding process between focal adhesion kinases with protein kinase B (Akt1), which is a target for potential cancer drugs. These developments include optimized probe and substrate functionalization processes and redesigned probe-substrate contact regimes. Furthermore, a statistical-based data processing method was developed to enhance the contrast of the experimental data. Collectively, these results demonstrate the potential of the AFM force spectroscopy in automating drug screening with high throughput.
Language英语
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/24733
Collection机器人学研究室
Corresponding AuthorYang, Ruiguo; Xi N(席宁)
Affiliation1.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48823 USA
2.Department of Biomedical Engineering, Michigan State University, East Lansing, MI 48823 USA
3.Department of Surgery, University of North Dakota, Grand Forks, ND 58202 USA
4.Department of Biomedical Sciences, University of North Dakota, Grand Forks, ND 58202 USA
5.Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong, Hong Kong, China
6.Department of Mechanicaland Materials Engineering, University of Nebraska Lincoln, NE 68588 USA
7.School of Information and Control Engineering, Shenyang Jianzhu University, Shenyang 110168, China
8.e State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110006, China
Recommended Citation
GB/T 7714
Yang YL,Zeng, Bixi,Sun, Zhiyong,et al. Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy[J]. IEEE TRANSACTIONS ON NANOTECHNOLOGY,2019,18:509-517.
APA Yang YL.,Zeng, Bixi.,Sun, Zhiyong.,Esfahani, Amir Monemian.,Hou, Jing.,...&Xi N.(2019).Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy.IEEE TRANSACTIONS ON NANOTECHNOLOGY,18,509-517.
MLA Yang YL,et al."Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy".IEEE TRANSACTIONS ON NANOTECHNOLOGY 18(2019):509-517.
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