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Biomimetic construction of peritoneum to imitate peritoneal metastasis using digital micromirror device-based optical projection lithography
Ge ZX(葛治星)1,2,3; Zhao JH(赵俊华)4,5; Yu HB(于海波)1,2; Yang WG(杨文广)6; Zhou PL(周培林)1,2,3; Wang ZN(王振宁)4,5; Liu LQ(刘连庆)1,2
Department机器人学研究室
Source PublicationLAB ON A CHIP
ISSN1473-0197
2020
Volume20Issue:17Pages:3109-3119
Indexed BySCI ; EI
EI Accession number20203609145508
WOS IDWOS:000562594400021
Contribution Rank1
Funding OrganizationNational Key R&D Program of China [2018YFB1304900] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [61727811, 61703395, 61803323, 81802900, 61821005] ; Major Scientific and Technological Special Project of Liaoning Province of China [2019JH1/10300007] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences [YJKYYQ20180027] ; Youth Innovation Promotion Association CAS [Y201943] ; LiaoNing Revitalization Talents Program [XLYC1807006]
Abstract

Currently, the mechanisms underlying the peritoneal metastasis of gastric cancer cells and the function of mesothelial cells during this process are unclear, primarily due to the absence of an effective in vitro peritoneal model. In this study, we constructed a biomimetic peritoneal model using a digital micromirror device-based optical projection lithography system. This model enabled the simulation of a damaged peritoneum, which allowed for a comparison of the characteristics of an undamaged peritoneum, such as porosity, mechanical properties, and surface morphology, with those of a damaged peritoneum. Biological inertness and removability of the polyethylene glycol dimethacrylate hydrogel were exploited to fabricate an arrayed heterogeneous interface that imitated a damaged human peritoneum. The porous structure of the peritoneum was achieved by adjusting the ratio of collagen I to gelatin methacryloyl; this structure of the peritoneum might contribute to its shock absorption property. Atomic force microscopy characterization showed that the outermost layers of the model peritoneum and real peritoneum were similar in surface morphology and mechanical properties. Furthermore, we reproduced the process of peritoneal metastasis in vitro. The numbers of gastric cancer cells that adhered to the heterogeneous interface were different, and mesothelial cells played an essential role in peritoneal metastasis. Our findings indicate that this model can be utilized in preclinical drug screening and personalized therapy.

Language英语
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation
WOS KeywordCANCER-CELLS ; MESOTHELIAL CELLS ; GASTRIC-CANCER ; ADHESION ; CHEMOTHERAPY ; DYNAMICS ; FIBROSIS ; BEHAVIOR ; PEGDMA ; SYSTEM
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation
Funding ProjectNational Key R&D Program of China[2018YFB1304900] ; National Natural Science Foundation of China[61727811] ; National Natural Science Foundation of China[61703395] ; National Natural Science Foundation of China[61803323] ; National Natural Science Foundation of China[81802900] ; National Natural Science Foundation of China[61821005] ; Major Scientific and Technological Special Project of Liaoning Province of China[2019JH1/10300007] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences[YJKYYQ20180027] ; Youth Innovation Promotion Association CAS[Y201943] ; LiaoNing Revitalization Talents Program[XLYC1807006]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/27571
Collection机器人学研究室
Corresponding AuthorYu HB(于海波); Wang ZN(王振宁); Liu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, China
2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China
3.University of Chinese Academy of Sciences, Beijing 100049, China
4.Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, Shenyang 110016, China
5.Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, Shenyang 110016, China
6.School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China
Recommended Citation
GB/T 7714
Ge ZX,Zhao JH,Yu HB,et al. Biomimetic construction of peritoneum to imitate peritoneal metastasis using digital micromirror device-based optical projection lithography[J]. LAB ON A CHIP,2020,20(17):3109-3119.
APA Ge ZX.,Zhao JH.,Yu HB.,Yang WG.,Zhou PL.,...&Liu LQ.(2020).Biomimetic construction of peritoneum to imitate peritoneal metastasis using digital micromirror device-based optical projection lithography.LAB ON A CHIP,20(17),3109-3119.
MLA Ge ZX,et al."Biomimetic construction of peritoneum to imitate peritoneal metastasis using digital micromirror device-based optical projection lithography".LAB ON A CHIP 20.17(2020):3109-3119.
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